Thermo Fisher Scientific LAP (Latency Associated peptide) Monoclonal Antibody (VB3A9), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

VB3A9

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866369

Product Specific Information

Description: This VB3A9 monoclonal antibody recognizes human latency-associated peptide (LAP), also known as the TGF-beta propeptide. Human LAP is a homodimer of 65-75 kDa that is non-covalently associated with TGF-beta and this complex is also referred to as the small latent complex (SLC). LAP can be covalently associated with latent TGF-beta binding proteins (LTBP) through disulfide bonds, forming the large latent complex (LLC). The association of LAP with TGF-beta facilitates its secretion and also renders TGF-beta inactive.

VB3A9 has been reported to cross-react with non-human primate LAP, but does not cross-react with mouse LAP.

Applications Reported: This VB3A9 antibody has been reported for use in immunoblotting (WB) and immunoprecipitation.

Applications Tested: This VB3A9 antibody has been tested by immunoblot analysis of human platelets under reducing conditions at 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

TGF beta-1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its prodomain. Activation of TGF-b1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF-beta, an aminoterminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.