Thermo Fisher Scientific TIGIT Monoclonal Antibody (MBSA43), Functional Grade, eBioscience

Thermo Fisher Scientific TIGIT Monoclonal Antibody (MBSA43), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• eFluor 450
• FITC
• NovaFluor Red 700
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM. This 26 kDa protein belongs to the poliovirus receptor (PVR) family. TIGIT is expressed on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but not on B cells or naive CD4+ T cells. Expression may be upregulated upon activation.

TIGIT interacts with members of the PVR and PVR-like families, including CD155, mediating interactions between NK/T cells and antigen-presenting cells, fibroblasts, and endothelial cells expressing PVR proteins.

MBSA43 is reported to cross-react with Rhesus macaque.

Applications Reported:

• Flow cytometric analysis
• Functional assay: enhances IFN-γ secretion following TCR activation

Applications Tested:

• Suitable for flow cytometry at ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• Antibody titration recommended for optimal performance.

Storage and Handling:

• Use in sterile environment.
• 0.2 µm post-manufacturing filtered.

Purity and Quality:

• Purity: >90% (SDS-PAGE)
• Endotoxin Level: <0.001 ng/µg (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

TIGIT binds with high affinity to the poliovirus receptor (PVR), leading to increased IL10 secretion, decreased IL12B secretion, and suppression of T cell activation through generation of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.