Thermo Fisher Scientific Phospho-p53 (Ser37) Polyclonal Antibody

Applications

• Western Blot (WB)
  Tested Dilution: 1:1,000

Product Specifications

Product Specific Information

• It is not recommended to aliquot this antibody.
• This antibody is not cross-reactive with p53 phosphorylated at other sites.

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or pyrimidine nucleotide starvation. p53 activates transcription of genes such as p21waf1, leading to G1 or G2/M arrest.
The structure of p53 includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization. Phosphorylation at Ser392 is associated with tumor formation. p53 also plays roles in aging, oxidative stress, and cognitive function. It is expressed at low levels in normal cells but highly in transformed cell lines, contributing to malignancy. Mutations in TP53 gene are common in cancers and can lead to loss of tumor suppressor activity. Germline mutations cause cancer-prone syndromes such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.