Thermo Fisher Scientific Phospho-STAT3 (Ser727) Polyclonal Antibody
STAT3 단백질의 Ser727 인산화를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, ICC/IF, IP, ChIP 등에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체. -20°C 보관, 방부제 무첨가.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | View 1 publication |
| Flow Cytometry (Flow) | - | View 1 publication |
| Immunoprecipitation (IP) | 1:50 | - |
| ChIP assay (ChIP) | 4 µL / 10⁶ cells | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer727 of mouse Stat3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 53 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES (pH 7.5), 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980044 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
STAT3 (Signal Transducer and Activator of Transcription 3) is a member of the STAT protein family. It is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for transcriptional activation by STAT3.
STAT3 can be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF, and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding. The STAT3 gene is located on chromosome 17q in humans. It plays a key role in processes such as cell growth and apoptosis. Rac1 regulates STAT3 activity, while PIAS3 acts as a specific inhibitor. Three alternatively spliced transcript variants encoding distinct isoforms have been identified. Deletion of the STAT3 gene in knockout mice is embryonically lethal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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