Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), NovaFluor Yellow 610, eBioscience

Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.8 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available

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Product Specific Information

The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally similar to MHC class I molecules. CD1d associates with beta2-microglobulin and functions analogously to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤ 0.8 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
  Optimal performance requires titration in the assay of interest.

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green

Filtration: 0.2 µm post-manufacturing filtered

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) provides improved formulation and is required for optimal staining with NovaFluor conjugates.

• Add 5 µL per sample before combining antibody cocktails with cells.
• For single-color controls: use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Internal testing shows non-specific staining of B cells in SJL mice, not observed in BALB/c or C57BL/6 strains.

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Target Information

CD1d is a heavy chain associated with beta-2 microglobulin on cortical thymocytes. It is the sole group 2 member of the CD1 family of MHC-like glycoproteins.
CD1 proteins present lipid and glycolipid antigens to T cells and differ by cellular localization and ligand specificity.
The CD1d gene product localizes to late endosomes and lysosomes via a tyrosine-based motif.
Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune disorders.

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For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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