Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), NovaFluor Red 685, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.6 µg/test

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Product Specifications

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Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

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Product Specific Information

The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. CD1d associates with beta2m and functions similarly to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes and recognizes beta2m-associated CD1d.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.6 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI. Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.

• Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures for engineered fluorescent signatures with minimized spillover and spread.

Excitation: 637 nm
Emission: 685 nm
Laser: 633–640 nm (Red)

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Target Information

CD1d is a heavy chain associated with β2-microglobulin on cortical thymocytes. It can also be expressed independently on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of MHC-like glycoproteins. These proteins present lipid and glycolipid antigens to T cells. The CD1 gene cluster resides on chromosome 1, differing in localization and ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail.

Associated Diseases: Mycobacterium malmoense, Autoimmune Disease

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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