Thermo Fisher Scientific Human BDNF Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

View 5 publications 5 publications

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

Human BDNF recombinant protein is a homodimer containing two 120 amino acid subunits linked by hydrophobic interaction

Molecular weight

27 kDa

Class

Recombinant

Type

Protein

Purity

≥98% by SDS-PAGE and HPLC

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 = 0.5 - 1.0 µg/mL; determined by the dose-dependent proliferation of rat C6 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute BDNF in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing carrier protein, such as 0.1% BSA and store in working aliquots from -20°C to -80°C.

Storage: The lyophilized protein is stable at room temperature for up to 1 month, but should be kept at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Target Information

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer`s and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.