Thermo Fisher Scientific Human Active BMP-7 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

CHO cells

Amino acid sequence

Human BMP-7 recombinant protein is a homodimer containing two 117 amino acid subunits (BMP-7, amino acids 315-431)

Molecular weight

28.8 kDa

Class

Recombinant

Type

Protein

Purity

≥98% by SDS-PAGE and HPLC

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 = 0.02 - 0.04 ng/mL; determined by the dose-dependent induction of alkaline phosphatase in mouse ATDC52 chondrogenic cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Carrier-Free

Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water, to a concentration of 0.1-1.0 mg/mL (Do not vortex) and store at 2-8°C for up to 1 week. Make any further dilutions of the reconstituted protein in buffer containing carrier protein, such as 0.1% BSA, and apportion into working aliquots to store at -20°C to -80°C.

Storage: The lyophilized protein is stable at room temperature for 1 month. Store the lyophilized protein at -20°C to -80°C,preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. Working aliquots stored with a carrier protein are stable for at least 7 months at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Target Information

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extra skeletal site. Based on its expression early in embryogenesis, BMP7 has a proposed role in early development. In addition, the fact that BMP7 is closely related to BMP5 and BMP6 has lead to speculation of possible bone inductive activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.