Thermo Fisher Scientific Human Midkine (MK) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

Human Midkine recombinant protein contains 123 amino acids

Molecular weight

13.4 kDa

Class

Recombinant

Type

Protein

Purity

>98% by SDS-PAGE

Endotoxin concentration

<0.1 ng/µg

Activity

ED50 = 0.1 - 10 ng/mL; determined by the dose-dependent chemotaxis of human neutrophils.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Wet or Dry ice (international)

Product Specific Information

Carrier-Free

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This lyophilized protein should be reconstituted in sterile, distilled water to a concentration of 0.1-1 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.

Target Information

Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.