Thermo Fisher Scientific CHRNA1 Polyclonal Antibody
Rabbit polyclonal antibody against human CHRNA1, validated for WB and IHC(P). Detects endogenous CHRNA1. Supplied as a liquid, 1 mg/mL, affinity purified, stored in PBS with glycerol and sodium azide. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CHRNA1 (Accession P02708), corresponding to amino acid residues E184–T234 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852112 |
Product Specific Information
Antibody detects endogenous levels of total CHRNA1.
Target Information
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that mediates the slower actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine.
The nAChRs fall into two subclasses: the muscle-type and the neuronal-type.
- Muscle-type receptor: Pentameric glycoprotein of five membrane-spanning subunits (2 alpha1, beta1, gamma or epsilon, and delta), forming a ligand-gated ion channel.
- Neuronal nAChR: Also a pentamer, but composed of alpha and beta subunits (alpha2–alpha8, beta2–beta4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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