Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (GT114)
다양한 종에 반응하는 알파 튜불린 단클론 항체로, Western blot, IHC, ICC/IF 등 여러 응용에 적합합니다. 고순도 Protein A 정제, 1 mg/mL 농도의 액상 형태로 제공되며, 세포골격 연구에 유용합니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:20,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly, Human, Mouse, Rat, Zebrafish |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT114 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787098 |
Additional Formats
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (96%), Xenopus laevis (95%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (100%).
- Positive Control: 293T, A431, HeLa, HepG2, NIH-3T3, mouse brain, PC-12, rat brain, drosophila, zebrafish eye, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, U87-MG.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha Tubulin forms a heterodimer with beta tubulin, each approximately 55 kDa in size. This heterodimer is the fundamental unit of microtubules, essential components of the eukaryotic cytoskeleton. Microtubules provide structural support, facilitate intracellular transport, and play a crucial role in mitosis.
Multiple alpha and beta tubulin genes are conserved across species. The alpha tubulin gene is homologous to the mouse and rat Tuba1 gene, with expression predominantly observed in differentiated neuronal cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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