Thermo Fisher Scientific UBA2 Recombinant Rabbit Monoclonal Antibody (JM66-53)
Recombinant rabbit monoclonal antibody against UBA2 for WB and IP applications. High specificity and sensitivity with consistent lot-to-lot performance. Produced in HEK293 cells, purified by Protein A, and supplied as a liquid form for research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
Immunoprecipitation (IP)
- Tested Dilution: 1:10–1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM66-53 |
| Immunogen | Synthetic peptide within Human SAE2/UBA2 aa 39–88 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2810049 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
UBA2 is involved in covalent attachment of one protein to another, a key posttranslational modification. Ubiquitin is well-known for this process, and SUMO (Sentrin) is a ubiquitin-like modifier implicated in stabilization and localization of target proteins.
SUMO conjugation requires the coordinated action of:
- E1-activating enzyme SAE1/SAE2
- E2-conjugating enzyme Ubc9
- E3-like SUMO ligase
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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