Thermo Fisher Scientific Phospho-HDAC4/HDAC5/HDAC9 (Ser246, Ser259, Ser220) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S(p)-EP) derived from Human HDAC4/HDAC5/HDAC9. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HDAC4/HDAC5/HDAC9,uniProtId:P56524-1,ncbiNodeId:9606,antigenRange:218-222|244-248|257-261,antigenLength:1084,antigenImageFileName:PA5-37835_HDAC4HDAC5HDAC9_P56524-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37835_HDAC4HDAC5HDAC9_P56524-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:HDAC4/HDAC5/HDAC9,uniProtId:Q9UKV0-1,ncbiNodeId:9606,antigenRange:218-222|244-248|257-261,antigenLength:1011,antigenImageFileName:PA5-37835_HDAC4HDAC5HDAC9_Q9UKV0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37835_HDAC4HDAC5HDAC9_Q9UKV0-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:HDAC4/HDAC5/HDAC9,uniProtId:Q9UQL6-1,ncbiNodeId:9606,antigenRange:218-222|244-248|257-261,antigenLength:1122,antigenImageFileName:PA5-37835_HDAC4HDAC5HDAC9_Q9UQL6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37835_HDAC4HDAC5HDAC9_Q9UQL6-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2554443

Product Specific Information

A suggested positive control for Western blot is 293 cells; suggested positive control for IHC is human breast carcinoma.

Target Information

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.