Thermo Fisher Scientific Phospho-PYK2 (Tyr580) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PTK2B(Accession Q14289), corresponding to amino acid residues around phosphorylated Tyr580. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PYK2,uniProtId:Q14289-1,ncbiNodeId:9606,antigenRange:580,antigenLength:1009,antigenImageFileName:PA5-99132_PYK2_Q14289-1_Rabbit.svg,antigenImageFileNamePDP:PA5-99132_PYK2_Q14289-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2818065

Product Specific Information

Antibody detects endogenous levels of PYK2 only when phosphorylated at Tyrosine 580.

Target Information

PTK2B (FAK2) is a cytoplasmic tyrosine kinase involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. PTK2B regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. FAK2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors, cytokine, chemokine, and growth factor receptors, and mediates responses to cellular stress. Aberrant FAK2 expression may play a role in cancer cell proliferation, migration, and invasion in tumor formation and metastasis. Elevated expression has been seen in gliomas, hepatocellular carcinoma, lung cancer, and breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.