Thermo Fisher Scientific SMAD3 Polyclonal Antibody
SMAD3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 IHC(P)에서 검증됨. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, 장기 보관 시 -20°C에서 보관 권장.
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Applications
Western Blot (WB)
- Tested Dilution: Not specified
- View 1 publication
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100
- View publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to internal region of human SMAD3 (aa 150–250) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1087333 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
Suggested positive control: normal breast or breast carcinoma.
Target Information
SMAD3 belongs to the SMAD family of proteins, similar to the gene products of the Drosophila mothers against decapentaplegic (Mad) and the C. elegans gene Sma.
SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD3 functions as a transcriptional modulator activated by transforming growth factor-beta and may play a role in carcinogenesis regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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