Thermo Fisher Scientific uNOS Polyclonal Antibody
uNOS Polyclonal Antibody는 iNOS, bNOS, eNOS를 모두 인식하는 Rabbit IgG 기반 항체입니다. Western blot 및 IHC(F/P) 분석에 적합하며, placenta 조직을 양성 대조로 사용 권장됩니다. 연구용으로만 사용되며, -20°C 이하에서 보관합니다.
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Thermo Fisher Scientific uNOS Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of mouse iNOS and nNOS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Gel ice packs |
| RRID | AB_2152743 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is placenta tissue.
Target Information
NOS oxidizes a guanidine nitrogen of arginine, releasing nitric oxide (NO) as a free radical and citrulline.
Nitric oxide acts as a messenger in various biological functions including:
- Vasodilation
- Neurotransmission
- Anti-tumor and anti-pathogenic activities
NOS is classified into three types:
- Neuronal NOS (nNOS or bNOS)
- Inducible NOS (iNOS or mNOS)
- Endothelial NOS (eNOS)
This antibody reacts with iNOS, bNOS, and eNOS.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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