Thermo Fisher Scientific STAT6 Polyclonal Antibody
STAT6 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, 인간 시료에 반응하며 WB, IHC, ChIP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상형 항체로 안정적인 실험 재현성 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:75 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human STAT6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.28 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2271213 |
Product Specific Information
This antibody is predicted to react with canine, mouse, porcine, and rabbit based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is placenta or tonsil tissue.
Target Information
STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL-13.
STAT6-deficient cells (Stat6-/-) show impaired IL-4-mediated functions, including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4-mediated proliferation.
STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta.
STAT6 is critically involved in Th2 immune response and exists in two isoforms: STAT6a and STAT6c.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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