Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, TRITC

Applications

Tested Dilution

Immunocytochemistry (ICC/IF)

4 µg/mL

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

TRITC TRITC TRITC

View additional formats

• [Alexa Fluor 350 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Donkey/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Donkey/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Donkey/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Donkey/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Donkey/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Donkey/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Donkey/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Donkey/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor 790/host/Donkey/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 405/host/Donkey/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 488/host/Donkey/)
• [Alexa Fluor Plus 555 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 555/host/Donkey/)
• [Alexa Fluor Plus 594 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 594/host/Donkey/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 647/host/Donkey/)
• [Alexa Fluor Plus 680 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 680/host/Donkey/)
• [Alexa Fluor Plus 800 ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Alexa Fluor Plus 800/host/Donkey/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/AP/host/Donkey/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/Biotin/host/Donkey/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/FITC/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L) Highly Cross-Adsorbed/conjugates/HRP/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

552/578 nm View spectra

Form

Lyophilized

Concentration

2.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2534714

Target

IgG

Cross Adsorption

Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep IgG

Antibody Form

Whole Antibody

Product Specific Information

Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. 1 year from date of receipt. Prepare working dilution prior to use and then discard. Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.