Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Secondary Antibody, FITC
Thermo Fisher Scientific의 Donkey anti-Sheep IgG (H+L) FITC 결합 2차 항체로, 면역세포염색(ICC/IF) 및 유세포분석(Flow Cytometry)에 적합합니다. FITC 형광(Ex/Em 498/517 nm)을 가지며, 높은 특이성과 감도를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534716 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- HRP
- TRITC
- Custom conjugation available upon request
Product Specific Information
- The sensitivity of each lot of antibody is confirmed using ELISA.
- The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Sheep secondary antibodies are affinity-purified reagents with well-characterized specificity for ovine (sheep) immunoglobulins. They are useful for detection, sorting, or purification of sheep antibodies.
Secondary antibodies provide versatility and increased sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These reagents are typically produced by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and optional modifications such as immunoaffinity chromatography, antibody fragmentation, or label conjugation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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