Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP
Western blot, IHC, ELISA에 적합한 Donkey anti-Rabbit IgG (H+L) AP 결합 2차 항체. 높은 교차흡착 처리로 비특이적 반응 최소화. 친화 크로마토그래피 정제 및 안정적 액상 제형. 연구용으로 다양한 검출 시스템에 활용 가능.
- 카탈로그번호
- A16038
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C (avoid freeze/thaw cycles). |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534712 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- Biotin
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies provide versatility, enabling various detection systems (e.g., HRP, AP, fluorescence) and improved sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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