Thermo Fisher Scientific 6x-His Tag Polyclonal Antibody
6x-His 태그 단백질 검출용 Rabbit Polyclonal 항체. Western blot, ICC/IF 등 다양한 응용 가능. 합성 펩타이드(HHHHHH) 면역원 사용. 항원 친화 크로마토그래피로 정제된 액상 형태. 연구용 전용 제품.
- 카탈로그번호
- PA1983B
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:250 | 16 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| Flow Cytometry (Flow) | - | 1 publication |
| ELISA | - | 2 publications |
| Immunoprecipitation (IP) | - | 3 publications |
| Competition Assay (CA) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues H H H H H H |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1069891 |
Product Specific Information
- PA1-983B detects 6x-His epitope tagged proteins.
- Successfully used in Western blot applications.
- Immunogen: synthetic peptide corresponding to residues H H H H H H.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminal of recombinant proteins to facilitate isolation or purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging enables detection of recombinant proteins by fusing a known epitope to the target protein, allowing identification even when no specific antibody exists.
His-tagged proteins bind strongly and selectively to Ni²⁺ ions, enabling purification by Ni²⁺-NTA affinity chromatography.
His tag-specific antibodies are widely used for detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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