Thermo Fisher Scientific CCL1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human I-309 (CCL1). Anti-Human I-309 (CCL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human I-309 (CCL1) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human I-309 (CCL1) (500-P110) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human I-309 (CCL1).

Western Blot:
To detect Human I-309 (CCL1) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human I-309 (CCL1) is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P110BT-1MG will be provided as 2 × 500 µg.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines, characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.