Thermo Fisher Scientific CCL1 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

View 1 publication 1 publication

In vitro Assay (IV)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human I-309 (CCL1) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL1,uniProtId:P22362-1,ncbiNodeId:9606,antigenRange:23-96,antigenLength:96,antigenImageFileName:500-P110BT-25UG_CCL1_P22362-1_Rabbit.svg,antigenImageFileNamePDP:500-P110BT-25UG_CCL1_P22362-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929253

Product Specific Information

AA Sequence of recombinant protein: SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human I-309 (CCL1). Anti-Human I-309 (CCL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human I-309 (CCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human I-309 (CCL1) (500-P110) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-309 (CCL1).

Western Blot: To detect Human I-309 (CCL1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human I-309 (CCL1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P110BT-1MG will be provided as 2 x 500 µg

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.