Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, PeproTech

Applications

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Human VEGF 165 matrix.

Sandwich ELISA:
To detect Human VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human VEGF 165 (500-P10GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human VEGF 165.

Western Blot:
To detect Human VEGF 165 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human VEGF 165 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system are fundamental regulators in the cell signaling of angiogenesis. Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine linked to this process.
Five different isoforms of VEGF have been described, generated by alternative splicing from a single VEGF gene. These isoforms bind to two tyrosine-kinase receptors, flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which are expressed almost exclusively on endothelial cells.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지