Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (STA), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

STA

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Biotin
• PE
• PE-Cyanine7
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802312

Product Specific Information

Description: The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: This STA antibody has been reported for use in flow cytometric analysis.

Applications Tested: This STA antibody has been pre-diluted and tested by flow cytometric analysis of stimulated HUVEC. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.