Thermo Fisher Scientific NCK2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 286 of Human Nck beta if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NCK2,uniProtId:O43639-1,ncbiNodeId:9606,antigenRange:1-286,antigenLength:380,antigenImageFileName:PA5-29210_NCK2_O43639-1_Rabbit.svg,antigenImageFileNamePDP:PA5-29210_NCK2_O43639-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546686

Product Specific Information

Recommended positive controls: A549, HepG2.

Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (83%), Pig (90%), Chicken (92%), Bovine (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.