Thermo Fisher Scientific PGP9.5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full length human UCHL1 purified from E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

30 mg/mL

Purification

IgY fraction

Storage buffer

PBS

Contains

10mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2942867

Product Specific Information

Specific for endogenous levels of the ~24 kDa UCHL1 protein.

Target Information

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.