Thermo Fisher Scientific MDM2 Monoclonal Antibody (IF2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 11 publications 11 publications

Immunohistochemistry (IHC)

-

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:200

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

1:25-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

1-5 µg/mL

-

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Bacteria, Guinea pig, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

IF2

Immunogen

Synthetic peptide derived from the n-terminal region of human MDM2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MDM2,uniProtId:Q00987-1,ncbiNodeId:9606,antigenRange:1,antigenLength:491,antigenImageFileName:33-7100_MDM2_Q00987-1_House_mouse.svg,antigenImageFileNamePDP:33-7100_MDM2_Q00987-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533136

Product Specific Information

This antibody recognizes the ~90 kDa (apparent MW) MDM2 protein. Also recognizes isoforms at ~57 and ~74/76 kDa. The epitope recognized by this antibody is located within amino acids 26-169 of the human protein. Positive controls: OsA-CL, MCF-7, HeLa cell lysates. HOS cells can be used for negative control. Staining of formalin fixed, paraffin embedded tissue requires heat induced epitope retrieval pretreatment.

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.