Thermo Fisher Scientific H-Ras Monoclonal Antibody (Y13-259)
H-Ras 단백질을 특이적으로 인식하는 Rat monoclonal antibody (clone Y13-259). Western blot, IHC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 고순도 affinity chromatography 정제. -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 2 publications |
| Immunohistochemistry (IHC) | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Y13-259 |
| Immunogen | Recombinant p21 ras protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533137 |
Target Information
Monomeric G protein Ras acts as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to downstream signaling pathways that lead to distinct cytoplasmic or nuclear events. Mammalian cells contain three Ras proto-oncogenes encoding closely related Ras proteins: H-, K-, and N-Ras. Oncogenic mutations in Ras genes occur in approximately 30% of human cancers. Constitutive activation of Ras due to mutations or overexpression promotes proliferation and inhibits apoptosis.
- K-Ras mutations: Common in pancreatic, colorectal, and non-small-cell lung carcinomas
- H-Ras mutations: Common in bladder, kidney, and thyroid carcinomas
- N-Ras mutations: Found in melanoma, hematological malignancies, and hepatocellular carcinoma
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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