Thermo Fisher Scientific p21 Monoclonal Antibody (EA10)
p21 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체 EA10. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. 인간 반응성, Mouse IgG1, kappa 아이소타입. 액상형, 0.5 mg/mL 농도, -20°C 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 9 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 2 publications |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EA10 |
| Immunogen | Full length recombinant human p21Cip1/WAF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533135 |
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. It plays a key role in the cellular response to genotoxic stress and is a major transcriptional target of p53. Nuclear p21 binds and inhibits Cdk1 and Cdk2, blocking the transition from G1 to S phase or G2 to mitosis after DNA damage, allowing DNA repair. In the cytoplasm, p21 has anti-apoptotic effects by inhibiting caspase 3 and apoptotic kinases ASK1 and JNK. p21 exhibits dual roles in carcinogenesis, functioning both as a tumor suppressor and as an oncogene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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