Thermo Fisher Scientific CD53 Recombinant Rabbit Monoclonal Antibody (104)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1:5,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 1–4 µL/mg of lysate

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to prevent contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal production candidates.

Advantages:

• Superior specificity and sensitivity
• Lot-to-lot consistency
• Animal-origin-free formulation
• Broader immunoreactivity due to larger rabbit immune repertoire

This antibody specifically recognizes Cynomolgus CD53.

Target Information

CD53 is a tetraspanin family transmembrane glycoprotein expressed in lymphoid-myeloid lineages. It forms complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4, and tetraspanins CD37, CD81, CD82, modulating signaling processes.

CD53 contributes to tumor cell radioresistance and exhibits anti-apoptotic effects. In thymus, CD53 is upregulated during T cell positive selection and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, while neutrophil stimulation leads to downregulation of CD53 expression.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.