Thermo Fisher Scientific 14-3-3 beta Monoclonal Antibody (05)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1:1,000-1:2,000

-

Immunoprecipitation (IP)

4-6 µL/mg of lysate

-

Product Specifications

Species Reactivity

Cynomolgus monkey

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

05

Immunogen

Recombinant Cynomolgus 14-3-3 beta/YWHAB protein (Met2-Asn244) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:14-3-3 beta,uniProtId:Q4R572-1,ncbiNodeId:9541,antigenRange:2-244,antigenLength:246,antigenImageFileName:MA5-28985_14-3-3_beta_Q4R572-1_House_mouse.svg,antigenImageFileNamePDP:MA5-28985_14-3-3_beta_Q4R572-1_House_mouse_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784925

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Cynomolgus 14-3-3 beta/YWHAB.

Target Information

14-3-3, a family of acidic and soluble proteins, highly conserved in amino acid sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms(beta, gamma, epsilon, eta, zeta, sigma and tau/theta) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.