Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), Brilliant Violet 480, eBioscience
인간 및 마우스 c-Maf 단백질을 인식하는 sym0F1 단클론 항체로, Brilliant Violet™ 480 형광체가 결합되어 Flow Cytometry에 최적화됨. 세포 내 염색에 사용되며, IL-4 및 IL-21 발현 연구에 유용. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 414-9855-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), Brilliant Violet 480, eBioscience
Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), Brilliant Violet 480
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution
- 5 µL (0.5 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet™ 480 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sym0F1 |
| Immunogen | Full-length E. coli-produced c-Maf |
| Conjugate | Brilliant Violet™ 480 |
| Excitation / Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3093966 |
Additional Formats
- Alexa Fluor™ 488
- Brilliant Violet™ 421
- eFluor™ 450
- eFluor™ 660
- PE
- PE-Cyanine7
- PE-eFluor™ 610
- PerCP-eFluor™ 710
- Custom conjugation available
Product Specific Information
Description:
The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems. In hematopoietic cells, c-Maf is crucial for IL-4 expression in Th2 cells and plays a role in Th17 and Tfh differentiation by regulating IL-21, IL-23R, and CXCR5 expression.
Applications Reported:
For intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-diluted and validated using mouse splenocytes and the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is compatible with cytometers equipped with a 405 nm violet laser. Use Brilliant Stain Buffer™ or Super Bright Complete Staining Buffer to minimize polymer dye interactions.
Not compatible with methanol-based fixation.
Laser Specifications:
Excitation: 440 nm
Emission: 479 nm
Laser: Violet
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
c-Maf is a basic leucine zipper transcription factor involved in Th2 differentiation, IL-10 expression, and lineage-specific hematopoiesis. It binds to Maf recognition elements (MARE) and regulates genes such as IL-4, insulin, and p53. Aberrations in maf are associated with multiple myeloma and expressed in myeloma cell lines and macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.