Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), Brilliant Violet 480

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution

• 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Alexa Fluor™ 488
• Brilliant Violet™ 421
• eFluor™ 450
• eFluor™ 660
• PE
• PE-Cyanine7
• PE-eFluor™ 610
• PerCP-eFluor™ 710
• Custom conjugation available

Product Specific Information

Description:
The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems. In hematopoietic cells, c-Maf is crucial for IL-4 expression in Th2 cells and plays a role in Th17 and Tfh differentiation by regulating IL-21, IL-23R, and CXCR5 expression.

Applications Reported:
For intracellular staining followed by flow cytometric analysis.

Applications Tested:
Pre-diluted and validated using mouse splenocytes and the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Violet™ 480 (BV480) emits at 479 nm and is compatible with cytometers equipped with a 405 nm violet laser. Use Brilliant Stain Buffer™ or Super Bright Complete Staining Buffer to minimize polymer dye interactions.
Not compatible with methanol-based fixation.

Laser Specifications:
Excitation: 440 nm
Emission: 479 nm
Laser: Violet

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

c-Maf is a basic leucine zipper transcription factor involved in Th2 differentiation, IL-10 expression, and lineage-specific hematopoiesis. It binds to Maf recognition elements (MARE) and regulates genes such as IL-4, insulin, and p53. Aberrations in maf are associated with multiple myeloma and expressed in myeloma cell lines and macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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