Thermo Fisher Scientific IL-6 Monoclonal Antibody (32C11)
마우스 IL-6 단백질을 인식하는 Rat IgG2a 단일클론 항체로, Western blot, ELISA, 중화 실험에 적합합니다. 비결합형 액상 형태이며, -20°C에서 보관합니다. 염증 및 면역 관련 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| ELISA | Assay-dependent | 4 publications |
| Neutralization (Neu) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 32C11 |
| Immunogen | Recombinant mouse IL-6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_223536 |
Product Specific Information
- MM600D targets IL-6 in ELISA and WB applications, showing reactivity with mouse samples.
- Immunogen: recombinant mouse IL-6.
- Detects IL-6 (~22 kDa).
- Has been paired as coating antibody in sandwich ELISA with detection antibody MM600CB (biotinylated clone 20F3).
- Typical dilutions: 1 µg/mL (coating), 0.125–0.25 µg/mL (detection).
- Used with recombinant mouse IL6 protein (SMIL6) in ELISA applications.
- Produced by injecting Rat IgG-secreting hybridoma cells into mice; antibody purified from ascites.
- Endotoxin level ≤10 EU/mg (LAL assay tested).
Target Information
IL-6 encodes a cytokine involved in inflammation and B cell maturation. It acts as an endogenous pyrogen, inducing fever in autoimmune or infectious conditions. IL-6 is mainly expressed at inflammation sites, secreted into serum, and activates transcriptional inflammatory responses via the IL-6 receptor alpha.
Elevated IL-6 levels are linked to inflammation-associated diseases such as diabetes mellitus, systemic juvenile rheumatoid arthritis, Kaposi sarcoma, and rheumatoid arthritis. Increased IL-6 expression has also been observed in viral infections, including COVID-19 (SARS-CoV-2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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