Thermo Fisher Scientific TRIM72 Monoclonal Antibody (A1D4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A1D4

Immunogen

Recombinant protein with Human TRIM72 aa 50-150 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRIM72,uniProtId:Q6ZMU5-1,ncbiNodeId:9606,antigenRange:50-150,antigenLength:477,antigenImageFileName:MA5-34958_TRIM72_Q6ZMU5-1_House_mouse.svg,antigenImageFileNamePDP:MA5-34958_TRIM72_Q6ZMU5-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848864

Product Specific Information

Positive Control: HCT116 cell lysates, rat skeletal muscle tissue, human pancreas tissue, A549.

Target Information

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.