Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (SPM589)
인간 p53 종양 억제 단백질을 검출하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 높은 특이성과 재현성을 제공하며, 인간 시료에 반응하고 마우스 및 랫과는 반응하지 않음. 연구용으로만 사용.
- 카탈로그번호
- 7157-MSM1X-Px (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific p53 Tumor Suppressor Protein Monoclonal Antibody (SPM589)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM589 |
| Immunogen | Recombinant human wild-type p53 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse or Rat.
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or pyrimidine nucleotide starvation. Upon stress, p53 up-regulates and activates transcription of genes such as p21waf1, leading to G1 or G2/M arrest.
Structurally, p53 contains:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerisation domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is critical for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells and functions as an anticancer agent. It is expressed at low levels in normal cells but highly in transformed cell lines, contributing to malignancy. Mutations in the TP53 gene result in loss of tumor suppressor activity and are common in various human cancers, including germline mutations seen in Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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