Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBioMM17F3), Functional Grade, eBioscience
IL-17A를 인식하는 마우스 단클론 항체로, 중화능을 가진 Functional Grade 제품입니다. 마우스 및 인간 시료에서 IL-17A 관련 연구에 적합하며, 낮은 엔도톡신 수준과 높은 순도를 제공합니다. 세포 기반 중화 및 기능 분석에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Flow Cytometry (Flow) | - | 1 publication |
| ELISA | - | 1 publication |
| Neutralization (Neu) | Assay-Dependent | 12 publications |
| Functional Assay | Assay-Dependent | 6 publications |
| Inhibition Assays (IA) | - | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMM17F3 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2866252 |
Product Specific Information
Description:
The eBioMM17F3 antibody is a mouse IgG1 monoclonal antibody that specifically reacts with mouse IL-17A and functions as a neutralizing antibody. It does not cross-react with other IL-17 family members (IL-17B, C, D, E, F). IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in conditions such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A enhances expression of ICAM-1 in fibroblasts and stimulates various cells (epithelial, endothelial, fibroblastic) to secrete IL-6, IL-8, G-CSF, and PGE2. It also supports proliferation of CD34+ progenitors and neutrophil maturation. IL-23-dependent Th17 cells producing IL-17A represent a distinct T helper subset regulated by IFN-γ and IL-4.
Applications Reported:
- Neutralization of mouse IL-17A bioactivity
- Intracellular staining of IL-17A-producing cells (IC Flow)
Applications Tested:
- Functional Grade Purified eBioMM17F3 antibody verified for low endotoxin level via LAL assay
- Tested for IL-17A bioactivity neutralization
In vitro, 8.0 ng/mL of this antibody neutralizes 50% of the biological effect of 5.0 ng/mL mouse IL-17A in an IL-6 secretion assay using NIH/3T3 cells.
Detailed cytokine bioassay and neutralization protocols are available in the BestProtocols® guide.
Storage and Handling:
- Use in a sterile environment
- 0.2 µm post-manufacturing filtered
Purity and Quality:
- Purity: >90% (SDS-PAGE)
- Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
- Aggregation: <10% (HPLC)
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory conditions.
It exists as a 32 kDa disulfide-linked homodimer and signals through IL-17R (CDw217). IL-17A induces expression of ICAM-1, IL-6, IL-8, G-CSF, PGE2, and nitric oxide, regulating NF-κB and MAPK pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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