Thermo Fisher Scientific IL-15 Monoclonal Antibody (AIO.3), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, lambda

Class

Monoclonal

Type

Antibody

Clone

AIO.3

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2866247

Product Specific Information

Description: The AIO.3 antibody reacts with mouse IL-15. This is a neutralizing antibody. Mouse IL-15 is a 14-15 kDa factor, previously known as IL-T. IL-15 is produced by a variety of cells and has a role in the T cell-mediated immune response and the central nervous system. IL-15 binds to the beta and gamma subunits of the IL-2 receptor; however, these two cytokines have distinct biological activities. IL-15 is a specific maturation factor for NK cells and mimics the BM microenvironment for NK cell development in vitro. It is reported that IL-15 delivers an anti-apoptotic and survival signal for NK, T, and B cells.

Applications Reported:The AIO.3 antibody is reported for use in neutralization of mouse IL-15 bioactivity.

Applications Tested: The Functional Grade Purified AIO.3 antibody has been tested by LAL assay to verify low endotoxin levels of < 0.01 ng/ug and in bioassay for neutralization of mouse IL-15 bioactivity.

The AIO.3 antibody at less than or equal to 0.39 µg/mL has been found to inhibit by 50% the biological effects of 50 ng/mL mouse IL-15 (14-8151) in a CTLL2 proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level:Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation:Less than 10%, as determined by HPLC.

Target Information

IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other`s activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.