Thermo Fisher Scientific RAN Polyclonal Antibody
RAN 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western Blot 및 IHC(Paraffin) 검증 완료. 합성 펩타이드(aa 197-216)를 면역원으로 사용하며, 비결합 액상 형태. 세포핵 내 RNA 및 단백질 이동 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 197–216 of human RAN protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550035 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is breast tissue.
Target Information
Ran (ras-related nuclear protein) is a small GTP-binding protein belonging to the RAS superfamily.
It is essential for translocation of RNA and proteins through the nuclear pore complex and is involved in control of DNA synthesis and cell cycle progression.
Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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