Thermo Fisher Scientific CD258 (LIGHT) Monoclonal Antibody (7-3 (7)), APC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The 7-3 (7) monoclonal antibody reacts with human CD258 (also known as LIGHT), a 29-kDa type II transmembrane protein in the TNF ligand superfamily related to lymphotoxin-β (LT-β). Expressed on activated T cells, particularly CD8+ T cells, LIGHT plays a role in cell survival, inflammation, and tumor eradication. Upon binding its ligand, herpesvirus entry mediator (HVEM), LIGHT induces a costimulatory effect on T cells, enhancing proliferation and cytokine production. LIGHT also indirectly interacts with the LT-β receptor.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis on PMA- and ionomycin-stimulated normal human peripheral blood cells. Recommended use at 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test).

Fluorophore Information:
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. It is induced by cytokines such as TNF and Fas ligand via death domain-containing receptors (TNFR1, Fas).
Death signals are transduced by DD-containing adapter molecules and members of the ICE/CED-3 protease family.
A novel DD-containing molecule, Daxx (death associated protein 6), binds specifically to Fas death domain, enhances Fas-induced apoptosis, and activates the Jun N-terminal kinase (JNK) pathway.
Daxx is widely expressed in fetal and adult human and mouse tissues, indicating its key role in Fas signaling pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지