Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), APC, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µL (1 µg)/test
• Publications: View 18 publications

Product Specifications

Available Formats

• Unconjugated
• APC-eFluor 780
• Biotin
• Brilliant UV 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. It is expressed predominantly on activated T and B lymphocytes.

PD-1 ligands include PD-L1 (B7-H1) and PD-L2 (B7-DC), which have overlapping functions and are expressed on normal tissues and activated antigen-presenting cells.

Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (Product # 11-9969).

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested for flow cytometry using stimulated normal human peripheral blood cells
• Recommended Use: 5 µL (1 µg) per test in a final volume of 100 µL
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

T-cell activation is regulated by inhibitory receptors such as PD-1 (PDCD-1), which bind to ligands PDL-1 and PDL-2 on antigen-presenting cells. Binding of PD-1 to its ligands inhibits immune activation in the absence of danger signals. PD-1-deficient mice exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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