Thermo Fisher Scientific Phospho-PKC alpha (Ser657, Tyr658) Polyclonal Antibody
인간 PKC alpha의 Ser657, Tyr658 인산화 부위를 인식하는 rabbit polyclonal antibody. Western blot 및 ELISA에 적합하며, 액상 형태로 제공. PBS/glycerol buffer에 보존되며 -20°C에서 보관. 세포 신호전달 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around S657 & Y658 of human PKC alpha (NP_0027282) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901867 |
Product Specific Information
- Positive Samples: 293T+CIP, 293T, 293T+PMA
- Immunogen sequence: GFSYVN
Target Information
The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in structure, tissue distribution, localization, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional PKC family, which are Ca²⁺- and phospholipid-dependent. PKC family members phosphorylate diverse protein targets and are involved in various cellular signaling pathways.
They also act as receptors for phorbol esters, known tumor promoters. PKC alpha plays roles in cell adhesion, transformation, cell cycle control, and cardiac contractility regulation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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