Thermo Fisher Scientific ATG5 Polyclonal Antibody
ATG5 단백질을 인식하는 Thermo Fisher Scientific의 양친매성 폴리클로날 항체. Western blot 및 IHC에서 사용 가능하며, 인간 시료 반응성. 합성 펩타이드 면역원 기반으로 제조되어 자가포식 및 세포사멸 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:2,000 |
| Immunohistochemistry (IHC) | 1:300–1:2,000 |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from human ATG5 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: ATG5.
Target Information
ATG5 (Autophagy Related 5) is an essential protein for autophagy and plays a role in apoptosis. It is involved in mitochondrial quality control after oxidative damage, negative regulation of innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation.
Upon conjugation to ATG12, the ATG5–ATG12 complex participates in autophagosome formation and contributes to autophagic cell death through interaction with FADD.
The conjugate forms a cup-shaped isolation membrane that detaches prior to or just after autophagosome completion.
ATG5 expression occurs downstream of caspase activity and associates with innate immune proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and facilitating viral replication.
Dysfunction of ATG5 is associated with spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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