Thermo Fisher Scientific DAD1 Polyclonal Antibody
DAD1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 적합합니다. Human과 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포사 억제 단백질 연구 및 세포사 메커니즘 분석에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to 14 amino acids near the C-terminus of human DAD1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152902 |
Product Specific Information
- Despite its predicted molecular weight, DAD1 often migrates at approximately 22 kDa in SDS-PAGE.
- Suggested positive control: HepG2 cell lysate.
- PA5-20062 can be used with blocking peptide PEP-0181.
Target Information
Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis.
DAD1 functions as a subunit of the mammalian oligosaccharyltransferase complex, essential for its structural integrity and function.
Mice lacking DAD1 exhibit abnormal N-linked glycoproteins and increased apoptosis-associated embryonic death.
Overexpression of DAD1 mRNA has been observed in certain human hepatocellular carcinomas, suggesting a potential role in carcinogenesis.
DAD1 is not a member of the inhibitor of apoptosis proteins (IAP) family and lacks baculoviral IAP repeat (BIR) domains.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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