Thermo Fisher Scientific H3K9me2 Polyclonal Antibody
H3K9me2 특이적 Rabbit Polyclonal 항체로 Western blot, ICC/IF, IP에 적합. 항원 친화 크로마토그래피로 정제되었으며, H3 Lys27 변형과 교차 반응하지 않음. -20°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:250 | View 2 publications |
| Immunoprecipitation (IP) | 1:25 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 in which lysine 9 is di-methylated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 165 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10986203 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with di-methylated or tri-methylated histone H3 Lys27.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow for the binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo several types of post-translational modification that influence cellular processes.
These include methylation or acetylation of lysine and arginine residues and phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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