Thermo Fisher Scientific H3K9acK14ac Polyclonal Antibody
H3K9acK14ac 다중 아세틸화 히스톤 H3를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP, ChIP 등 다양한 응용에 사용 가능. 높은 특이성과 재현성을 제공하며, 인간 및 동물 시료에 반응. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:800–1:3,200 | - |
| Immunoprecipitation (IP) | 1:50 | - |
| ChIP assay (ChIP) | 1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat, Yeast |
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the amino terminus of histone H3 acetylated on lysines 9 and 14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 309 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154175 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other acetylated histones.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow for the binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo post-translational modifications such as methylation, acetylation, and phosphorylation, influencing key cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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