Thermo Fisher Scientific Phospho-p27 Kip1 (Thr198) Polyclonal Antibody
인간 p27 Kip1 (Thr198) 인산화 형태를 인식하는 Rabbit Polyclonal 항체로, 세포주기 조절 연구에 적합합니다. Dot blot에서 1:500 희석으로 검증되었으며, Protein A 및 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, 장기 보관 시 -20°C 권장.
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Applications
- Dot blot (DB)
Tested Dilution: 1:500
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T198 of human p27Kip1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2078008 |
Target Information
p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFβ-induced G1 arrest. It is an important regulator of cell cycle progression and a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Depending on its phosphorylation state and stoichiometry, p27 Kip1 acts either as an inhibitor or activator of cyclin type D-CDK4 complexes. It has a molecular weight of approximately 27 kDa, and its degradation, triggered by CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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