Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), APC-eFluor 780, eBioscience

Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), APC-eFluor 780

Applications

• Tested Application: Flow Cytometry
• Recommended Dilution: 5 µL (0.25 µg)/test
• Publications: 21 publications available

Product Specifications

Product Specific Information

The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed on developing and mature B cells but not plasma cells. Low levels may be found on a subset of mature T cells. CD20 is implicated in B-cell activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells
• Recommended Usage: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorophore Characteristics:
APC-eFluor 780 emits at 780 nm and is excited by the red laser (633 nm). Ensure instrument compatibility.

Light Sensitivity:
Sensitive to photo-induced oxidation; protect vial and stained samples from light.

Fixation Guidance:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation/Emission: 633–647 nm / 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD20 is a non-glycosylated surface phosphoprotein (~33–37 kDa) expressed on mature and most malignant B cells, a subset of T lymphocytes, and follicular dendritic cells. It regulates transmembrane calcium conductance, cell cycle progression, and B-cell proliferation. CD20 is associated with lipid rafts and colocalizes with BCR upon receptor ligation but remains on the cell surface after internalization events. CD20 is a key target for antibody-mediated B-cell depletion therapies.

For Research Use Only. Not for use in diagnostic procedures.

Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC
• Biotin
• Brilliant UV 395, 496, 615, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 610-70S, 660-120S
• NovaFluor Red 710, 725
• NovaFluor Yellow 590, 660
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available upon request

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