Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), eBioscience
인간 CD62L(L-Selectin)을 인식하는 DREG-56 단클론 항체로, 유세포분석, Western blot, 면역조직화학 등 다양한 응용에 사용됩니다. 0.5 mg/mL 농도의 액상 형태이며, PBS(pH 7.2)에 0.09% sodium azide가 포함되어 있습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 14-0629-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 10 publications |
| Neutralization (Neu) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | 2 publications |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DREG-56 (DREG56) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467319 |
Available Conjugate Formats
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Brilliant UV 496 / 615 / 737 / 805
- Brilliant Violet 421 / 480 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue (510, 555, 585, 610-30S, 660-40S, 690, 760)
- NovaFluor Red (710, 725, 755)
- NovaFluor Yellow (570, 590, 660)
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600
- Custom conjugation available upon request
Product Specific Information
Description:
The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, and sulfated sialylated glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. These interactions mediate lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.
Applications Reported:
- Flow cytometric analysis
- Non-reducing Western blotting
- Immunohistology of frozen tissue sections
- Inhibition of binding to HEV (use Functional Grade DREG-56, Product #16-0629-85)
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤1 µg/test in 100 µL volume. Cell number should be optimized (10⁵–10⁸ cells/test). Antibody should be titrated for best performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor. It includes:
- C-type lectin binding domain
- EGF-like domain
- Two short consensus repeat (SCR) sequences
- Spacer, transmembrane, and cytoplasmic regions
It is constitutively expressed on most leukocytes including lymphocytes (except many memory T cells), monocytes, and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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