Thermo Fisher Scientific CD24 Monoclonal Antibody (30-F1), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD24 Monoclonal Antibody (30-F1), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• FITC
• PE
• Request custom conjugation

Product Specific Information

The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane and expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage.
CD24 expression detected by 30-F1 helps resolve stages of B lymphopoiesis in mouse bone marrow. P-selectin (CD62P) binds to CD24, and glycosylation heterogeneity causes variable molecular mass and staining intensity among lineages.

Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse splenocytes, ≤1.0 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm, designed for cytometers with ultraviolet (355 nm) laser. Ensure instrument compatibility.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation Compatibility:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).
Compatible with short-term methanol fixation (<1 hour). Avoid long-term methanol storage.

Excitation/Emission:

• Excitation: 355 nm
• Emission: 797 nm
• Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen.

Target Information

CD24 is a GPI-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells to mature B cells. Expression is low or absent in plasma cells. CD24 is also found in most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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