Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PerCP-eFluor 710

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 11 publications

Product Specifications

Additional Formats:

• Unconjugated
• Alexa Fluor 488
• Biotin
• eFluor 660
• PE
• Custom conjugation available

Product Specific Information

The eBioMC-480 (MC-480) antibody reacts with stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed on early mouse embryos, murine embryonal carcinoma cells (EC), embryonic stem cells (ES), and germ cells (EG).
No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC increases SSEA-1 expression, while in mouse it is diminished.
SSEA-1 is associated with cell adhesion, migration, and differentiation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated on F9 cells at 5 µL (0.125 µg) per test.
A test = antibody amount that stains a cell sample (100 µL total volume).
Cell number range: 10⁵–10⁸ cells/test.

Fluorochrome Information:

• PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
• Emission: 710 nm; Excitation: 488 nm (Blue laser).
• Recommended filters: 685 LP dichroic mirror + 710/40 band pass filter (alternative: 695/40 BP).
• Stable after fixation with freshly prepared 2% formaldehyde overnight at 4°C.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); SSEA-1)
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous system, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X.
Cells with high Le(x) expression exhibit calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression aids in Hodgkin’s disease diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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